TABLE 1.
Comparison of Meridian illumigene C. difficile and Cepheid Xpert C. difficile/Epi assays for detection of C. difficile RT033a
| Ribotype (strain) | No. tested | Toxin profile | Toxinotype(s) | Source (no. of isolates) | Xpert detection of: |
Xpert results | illumigene result | ||
|---|---|---|---|---|---|---|---|---|---|
| tcdB | CDT | tcdC | |||||||
| Test strains | |||||||||
| RT033 | 52 | A− B− CDT+ | Xla, Xlb | Human (15) | NEG | POS | NEG | Toxigenic C. difficile NEG; presumptive RT027 NEG | NEG |
| Bovine (21) | |||||||||
| Porcine (16) | |||||||||
| RT017 | 4 | A− B+ CDT− | VIII | Human | POS | NEG | NEG | Toxigenic C. difficile POS; presumptive RT027 NEG | POS |
| RT237 | 4 | A− B+ CDT+ | XXXI | Human (2) | POS | POS | NEG | Toxigenic C. difficile POS; presumptive RT027 NEG | NEG |
| Porcine (2) | |||||||||
| RT239 | 1 | A− B− CDT+ | NA | Human | NEG | POS | NEG | Toxigenic C. difficile NEG; presumptive RT027 NEG | NEG |
| RT280 | 1 | A− B+ CDT+ | XXX | Human | POS | POS | NEG | Toxigenic C. difficile POS; presumptive RT027 NEG | NEG |
| RT281 | 1 | A− B+ CDT+ | XXX | Human | POS | POS | NEG | Toxigenic C. difficile POS; presumptive RT027 NEG | NEG |
| Reference strains | |||||||||
| RT087 (VPI 10463) | 1 | A+ B+ CDT− | 0 | Human | POS | NEG | NEG | Toxigenic C. difficile POS; presumptive RT027 NEG | POS |
| RT033 (IS 58) | 1 | A− B− CDT+ | Xla | Human | NEG | POS | NEG | Toxigenic C. difficile NEG; presumptive RT027 NEG | NEG |
| RT012 (CD630) | 1 | A+ B+ CDT− | 0 | Human | POS | NEG | NEG | Toxigenic C. difficile POS; presumptive RT027 NEG | POS |
| RT027 (R 20291) | 1 | A+ B+ CDT+ | III | Human | POS | POS | POS | Toxigenic C. difficile POS; presumptive RT027 POS | POS |
| RT010 (CD062) | 1 | A− B− CDT− | NA | Human | NEG | NEG | NEG | Toxigenic C. difficile NEG; presumptive RT027 NEG | NEG |
a
POS, positive; NEG, negative; NA, not applicable (no pathogenicity locus present).