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. 2015 Feb 19;53(3):830–837. doi: 10.1128/JCM.02648-14

TABLE 1.

RT-RPA primers and exo probe used for the rapid detection of DENV

Name Sequence (5′ to 3′)a
DenMPF19PS CAGCATATTGACGCTGGGAGAGACCAGAGATC*C
DenMPR11PS GAACCTGTTGATTCAACAGCACCATTCCATTT*T
DenMPR32PS GAACCTGTTGGATCAACAACACCAATCCATC*T
DenMPExoP9FAM CCATTTTCTGGCGTTCTGTGCCTGGAATGATG(dT-FAM)TG(dSpacer)(dT-BHQ1)GAGACAGCAGGAT-3′C3-Spacer
a

Asterisks indicate a phosphothioate link in the backbone to inhibit exonuclease degradation; dT-FAM, thymidine nucleotide carrying fluorescein; dSpacer, tetrahydrofuran basic site mimic; dT-BHQ1, thymidine nucleotide carrying black hole quencher 1; 3′C3-Spacer, C3 spacer at the 3′ end to block elongation.