TABLE 2.
Summary of dengue detection in serum samples (n = 203) from patients with clinically suspected dengue at UMMC using RT-RPA, RT-LAMP, and qRT-PCR assays and IgM and IgG ELISA
| Assay and results (no. of tests) | IgM ELISA results (no. [%]): |
IgG ELISA results (no. [%]) |
||
|---|---|---|---|---|
| Positive (n = 91) | Negative (n = 112) | Positive (n = 96a) | Negative (n = 107) | |
| RT-RPA | ||||
| Positive (50b) | 17 (34.0) | 33 (66.0) | 16 (32.0) | 34 (68.0) |
| Negative (153) | 74 (48.4) | 79 (51.6) | 80 (52.3) | 73 (47.7) |
| RT-LAMP | ||||
| Positive (51c) | 13 (25.5) | 38 (74.5) | 14 (27.5) | 37 (72.5) |
| Negative (152) | 78 (51.3) | 74 (48.7) | 82 (53.9) | 70 (46.1) |
| qRT-PCR | ||||
| Positive (61) | 22 (36.1)d | 39 (63.9)d | 22 (36.1)e | 39 (63.9) |
| Negative (142) | 69 (48.6)d | 73 (51.4) | 74 (52.1) | 68 (47.9) |
a
Sixteen IgG-positive dengue samples tested negative by qRT-PCR and dengue IgM ELISA.
b
Three RT-RPA-positive samples tested negative by qRT-PCR.
c
Five RT-LAMP-positive samples tested negative by qRT-PCR.
d
Acute DENV infection was confirmed in 130 samples (dengue IgM- or qRT-PCR-positive samples).
e
Eight dengue IgG- and qRT-PCR-positive samples tested negative by dengue IgM ELISA.