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Shigella biomarker development. A gel electrophoresis image of amplicons from the 5 major clades identified in this study (lanes 1 to 5) is shown; two bands, one genus targeted (ipaH3) and one clade targeted (S1 to S5), indicate a positive reaction. Lane 6 shows a coinfection reaction with all 5 clades, plus the universally conserved ipaH3 marker. Numbers on the left represent numbers of base pairs in the DNA ladder.
