TABLE 1.
Virulence gene targets and oligonucleotide sequences used in the quantitative PCR microarray
| Virulence gene targets | Forward primer/reverse primer |
|---|---|
| eae | CATTGATCAGGATTTTTCTGGTGATA/CTCATGCGGAAATAGCCGTTA |
| eae-gamma | GACTGTTAGTGCGACAGTCAGTGA/TTGTTGTCAATTTTCAGTTCATCAAA |
| ecs1763 | TACTCTTGACCTTAGTACCGC/TTTTGATTATCAGAACCATATAGCCC |
| ecs1822 | CCGTACAGCGTGATTCATCC/TAGCGAAACGGGCAAGGTC |
| efa1 | TTTTCACCAGTTCATCATACAGG/CCATTATAAACATTTGCCAGACC |
| efa2 | ACTAAGATCAATACAAGGATTCC/ATCCATCAGGCCATAGGTG |
| ehxA | CGTTAAGGAACAGGAGGTGTCAGTA/ATCATGTTTTCCGCCAATGAG |
| ent | TCCTGGATTATTTTCTGCATTTCA/ACTATTGCCAAGTACGCCACAA |
| espF1 | TCGYCCGGCMCCRCCG/TGCCTGWGCAATGGGCGG |
| espG | AGCTGAAGTTGTGGARTTTTTATGC/TGTGTCAACRTTAAGGCTGGCA |
| espJ | AAAGGAGCMAAAGTATATCCMGATA/AACATCSASYCTRACTGWTTCTGG |
| espK | GCAGRCATCAAAAGCGAAATCACACC/TCGTTTGGTAACTGTGGCAGATACTCG |
| espM1 | TCAGCTCTTTTGGTATCA/CGCTAAATTTGTTAACATTAAAG |
| espM2 | CAGCACAAAGTTCTATTAATCATGTRATAATGGGG/ACTTGTCCGCAAGCAAGTTTGCTATG |
| espN | GACATATTTGTTTATGTCATCAGGAGCGG/CCTCAGGATATGGATGGCCTACTGGC |
| espO1-1 | CATGTTGTTGATGTAAGTATGCAG/AAGTTCACAAGTACATTACCCGG |
| espP | ATGCCCCGTCAGCATCTG/TCGACCGTCAGCGTATGG |
| espR1 | GCTCATGTTATTTAATTTRTTTTCCC/AGTGAAACAATTCGTTATCCACATC |
| espV | TCAGGTTCCTCGTCTGATGCCGC/CTGGTTCAGGCCTGGAGCAGTCC |
| espW | CCAAACTTAGGAGAGCGAGACGTAAGAATG/CCTGAATAAAGATACTCACCTAACTCTG |
| espX1 | ACTCAACTTGTCAATAAGCACTTAGG/ACACATCATTATTTAGCTTTACATTGTC |
| espX2 | TGTTATCATCCGATAACTCTGGG/GTCTTTTTCTTAACCTCTGGCTC |
| espX6 | TCACAACCGTGATTCTATATGAAAC/TTAGTATTGCGCCAGTAAGAATTAC |
| espX7 | TGCTGAAGAATTGAATTACTCT/TGTATCATCAAGCACTGCTCC |
| espY1 | ACTTCTGTTCAATTTCGTGAATAAAA/ATTCCAGACTATGGATAAACTTTTCTT |
| espY3 | GAACTATCTCTATTGTTAACGAGG/CTAGATATAGACCGCTGAAATCG |
| espY4-2 | GCAATGTATAAGGAGAGGCTTAGTC/TTGACTTCTTTAATATGAGAGCCAGG |
| etpD | TTGGATGACGGCGAAACTG/AGATGATACGCTGTTGGGAG |
| iha | AGTGGTACGGGTAAAACCG/AGTATCAGCGTGTAACTGGC |
| katP | GAAGTCATATATCGCCGGTTGAA/GTCATTTCAGGAACGGTGAGATC |
| lpfA | TACTGTCCGTTGACTCTCAG/ACCAACCGCAGCAAATACAG |
| nleA | AGATAACYCTAATACTAAATATGCC/GCCCAACCATTGCRCCGATATGAGG |
| nleB | CATGTTGAAGGCTGGAASTTTGT/CCGCTACAGGGCGATATGTT |
| nleD-2 | GGACTGGTTCCGATTTTCACTG/AAAGTCCACCACGCTAATCCC |
| nleE | AGAAGCGTTTGAACCTATTTCCA/TTGGGCGTTTTCCGGATAT |
| nleF | TGAGGTGAGAAATGAAAATACTGATG/CTATCCCTGTCCTCTATCGTCATTC |
| nleG | CCAGATTTTCTGCGAGAAGGC/GGACAGTTTTAGCGTGGAACC |
| nleH1-1 | TACCGAGTGTGGACTATA/CAGGACTTTTGTTGCATC |
| nleH1-2 | ACAAGAGAAAGTCATAGTGGTTG/AATCTCYCCCTTAGGCCATCCCA |
| pagC | GGCTGATAATCATACGCTATCG/ATCGATATTGCAGATTCACTCC |
| stx, all variants | TTTGTYACTGTSACAGCWGAAGCYTTACG/CCCCAGTTCARWGTRAGRTCMACRTC |
| terE | GCCGTTACCATCTATGATGC/TGTAAACGCGCATGAAGCTG |
| toxB | AGTATCAGTCACATAAAGTAGAC/GCATTRGGATCAATCCAGAG |
| ureD | GCAATAATTGACTCTGATTGCC/GCTGCTGCGGTAAAATTTACT |