Table 2. Bacterial strains used for preliminary validation of the real-time-tpi-tcdA-tcdB-cdtA PCR assay.
| Organisms | Multiplex PCR results* | |||
|---|---|---|---|---|
| tpi | tcdA | tcdB | cdtA | |
| Clostridium difficile R20291 (BI/NAP1/027), C. difficile CD196 (BI/NAP1/027), C. difficile M120 (BK/NAP7,8,9/078), C. difficile CD305 (023) | + | + | + | + |
| C. difficile ATCC 9689, C. difficile BI-9 (J/NAP2/001), C. difficile Liv024 (J/NAP2/001), C. difficile Liv022 (DH/NAP11/106), C. difficile TL178 (G/NAP6/002), C. difficile TL176 (Y/NAP4/014), C. difficile TL174 (015), C. difficile 630 (R/012) | + | + | + | - |
| C. difficile CF5 (CF/NAP9/017), C. difficile M68 (CF/NAP9/027) | + | - | + | - |
| Klebsiella pneumoniae ATCC 13883, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, Campylobacter jejuni ATCC 33291, Enterococcus casseliflavus ATCC 700327, Streptococcus pyogenes ATCC 19615, Enterobacter aerogenes ATCC 13048, Acinetobacter anitratus ATCC 19606, Salmonella typhi ATCC 14028, Shigella flexneri ATCC 12022, Enterococcus faecalis ATCC 29212, Acinetobacter baumannii BAA 747, Streptococcus pneumoniae ATCC 46619 | - | - | - | - |
*tpi, Clostridium difficile specific triose phosphate isomerase gene; tcdA, toxin A gene; tcdB, toxin B gene; cdtA, binary toxin gene.
+, positive result; -, negative result.