Table 3. Statistical data of hematologic parameters between hemolyzed and non-hemolyzed specimens.

Hematologic parameters (unit)	P value by Kolmogorov-Smirnov test		Comparison of mean by paired t-test
	[B - A]*	[C - A]†	[B - A]			[C - A]
			Mean	SD	P	Mean	SD	P
WBC (109/L)	0.102	0.200	-0.17	0.35	0.006	0.05	0.23	0.185
RBC (1012/L)	0.175	0.200	-0.14	0.19	< 0.001	-0.70	0.51	< 0.001
mHb (g/dL)	0.051	0.006	0.11	0.39	0.111	-0.14	0.53	0.121
Hct (%)	0.129	0.200	-2.29	2.04	< 0.001	-7.46	4.57	< 0.001
MCV (fL)	0.063	0.016	-1.91	0.70	< 0.001	-2.41	1.54	< 0.001
MCH (pg)	0.081	0.146	1.27	1.19	< 0.001	5.68	4.55	< 0.001
MCHC (g/dL)	0.050	0.037	2.04	1.33	< 0.001	6.67	5.24	< 0.001
CHCM (g/dL)	0.001	0.030	1.08	0.55	< 0.001	1.11	0.76	< 0.001
CH (pg)	< 0.001	< 0.001	0.32	0.53	0.001	0.18	0.49	0.028
RDW (%)	0.001	0.001	-0.29	0.43	< 0.001	-0.27	0.46	0.001
HDW (g/dL)	< 0.001	0.032	-0.01	0.12	0.448	0.01	0.16	0.761
PLT (109/L)	0.200	0.146	-15.78	13.32	< 0.001	-4.78	22.99	0.214
MPV (fL)	< 0.001	0.008	0.12	0.88	0.396	0.23	0.99	0.172
Hyper (%)	< 0.001	< 0.001	0.87	0.82	< 0.001	0.96	1.08	< 0.001
Hypo (%)	0.001	< 0.001	-0.39	0.50	< 0.001	-0.38	0.52	< 0.001
Macro (%)	0.032	0.139	-0.54	0.52	< 0.001	-0.64	0.62	< 0.001
Micro (%)	< 0.001	0.001	0.08	0.10	< 0.001	0.11	0.11	< 0.001
RBC Fragments (1012/L)	< 0.001	< 0.001	0.00	0.00	0.003	0.00	0.00	0.017
RBC Ghosts (1012/L)	< 0.001	0.009	0.01	0.01	< 0.001	0.02	0.01	< 0.001
MN (%)	0.200	0.200	-2.44	2.27	< 0.001	-4.79	5.24	< 0.001
PMN (%)	0.200	0.200	2.30	2.18	< 0.001	3.98	5.24	< 0.001
cHb (g/dL)	0.200	0.200	-0.32	0.68	0.007	-2.14	1.70	< 0.001
NEUT (%)	0.200	0.002	0.88	1.62	0.003	1.34	3.16	0.014
LYMPH (%)	0.128	0.006	-0.76	1.57	0.007	-0.61	6.32	0.560
MONO (%)	0.200	0.200	0.05	0.73	0.662	0.19	0.73	0.114
EOS (%)	0.004	0.006	0.06	0.65	0.604	-0.02	0.59	0.802
BASO (%)	0.040	0.031	-0.13	0.32	0.022	-0.02	0.36	0.786
LUC (%)	0.087	0.200	-0.10	0.47	0.202	-0.02	0.54	0.856
LI	0.200	0.200	-0.01	0.12	0.737	-0.03	0.16	0.204
MPXI	< 0.001	0.023	-1.26	1.63	< 0.001	-1.75	2.07	< 0.001
WBCP (109/L)	0.200	0.143	-0.18	0.36	0.007	-0.29	0.35	< 0.001
RETIC-P (%)	0.200	0.043	-0.24	0.17	< 0.001	-0.22	0.35	0.001
RETIC-N	0.200	0.062	-13.7	8.79	< 0.001	-19.40	22.55	< 0.001
CHr (pg)	< 0.001	< 0.001	0.72	1.07	0.001	0.59	1.07	0.004
CHm (pg)	< 0.001	< 0.001	0.66	1.06	0.001	0.49	1.04	0.012
mHb-cHb (g/dL)	0.166	0.087	0.42	0.49	< 0.001	2.01	1.48	< 0.001
MCHC-CHCM (g/dL)	0.077	0.046	0.96	1.22	< 0.001	5.55	5.20	< 0.001

^*[B-A] is defined as the data set obtained by subtracting the results of aliquot A from those of aliquot B; ^†[C-A] is defined as the data set obtained by subtracting the results of aliquot A from those of aliquot C.

Abbreviations: WBC, white blood cell; RBC, red blood cell; mHb, hemoglobin concentration measured by the colorimetric method; MCV, mean cell volume; MCH, mean cell hemoglobin; MCHC, mean cell hemoglobin concentration; CHCM, cell hemoglobin concentration mean; CH, cellular hemoglobin content (mean of RBC cellular hemoglobin histogram); RDW, red cell distribution width; HDW, hemoglobin distribution width; PLT, platelet; MPV, mean platelet volume; Hyper, hyperchromia; Hypo, hypochromia; Macro, macrocytosis; Micro, microcytosis; MN, mononuclear cell; PMN, polymorphonuclear cell; cHb, calculated hemoglobin (CHCM×RBC×MCV÷1,000); NEUT, neutrophil; LYMPH, lymphocyte; MONO, monocyte; EOS, eosinophil; BASO, basophil; LUC, large unstained cell; LI, lobularity index; MPXI, myeloperoxidase index; WBCP, white blood cell count by peroxidase; RETIC-P, reticulocyte percent; RETIC-N, reticulocyte number; CHr, cellular hemoglobin mean of reticulocyte; CHm, cellular hemoglobin mean of mature RBC.