Skip to main content
. 2015 Apr 7;108(7):1683–1696. doi: 10.1016/j.bpj.2015.02.018

Figure 5.

Figure 5

NCA SSNMR spectra of (A) PL-ASR, (B) rbUCN EM-ASR, and (C) rbUCN EM-NIC. All peaks are labeled according to their assignments in PL-ASR. All spectra were recorded on an 800 MHz spectrometer and processed identically using 15 Hz of Lorentzian line narrowing and 30 Hz of Gaussian line broadening in the indirect dimension, and 30 Hz of Lorentzian line narrowing and 60 Hz of Gaussian line broadening in the direct dimension. The spectrum in (A) was collected with 32 scans, while spectra (B) and (C) were both collected with 320 scans. In (A), the lowest contour is cut at 10×σ, while in (B) and (C) the lowest contour level is cut at 5×σ. Representative overlays of two-dimensional planes of (D–G) three-dimensional NCACB and (H) CANCO experiments performed on PL-ASR (red), EM-ASR (black), and EM-NIC (blue). All peaks are labeled according to the assignments. PL-ASR and EM-NIC spectra were recorded on an 800 MHz spectrometer while EM-ASR spectra were recorded on a 900 MHz spectrometer. Additional planes are shown in Fig. S4. To see this figure in color, go online.