Figure 6.

Distance distributions between E771 and N796 in E1⋅H⁺₇₇₁-PLB. C_δ-C_δ distance distributions between residues E771 and N796 were calculated for each trajectory (MD1–MD3) of E1⋅H⁺₇₇₁-PLB. Distance distributions were fitted to a two-Gaussian distribution. The dashed line indicates the distance cutoff at which E771 and N799 are bound by at least one hydrogen bond (R ≤ 0.5 nm). Distances longer than this cutoff value indicate the absence of a hydrogen bond. pKa values (mean ± SE) for E771 in the presence and absence of hydrogen bonds are shown in the plots. To see this figure in color, go online.