Table 2.
Important EGFR binding partners in HEK 293 cells according to each of four different metrics
| Simulation | Copy number (#) | KD | Ratio (#/KD) |
|---|---|---|---|
| SHC1 | TXN | SHC1 | SHC1 |
| PLCG1 | CRKL | SHC2 | GRB2 |
| GRB2 | GRB2 | PIK3R2 | SH2B3 |
| SH2B3 | CRK | SOCS6 | PLCG1 |
| SRC | PTPN11 | RASA1 | PTPN11 |
| RASA1 | SH2B3 | PIK3R3 | PIK3R2 |
| PTPN11 | SUPT6H | PLCG1 | RASA1 |
| YES1 | PLCG1 | GRAP2 | SRC |
| PIK3R2 | NCK1 | SH2B3 | YES1 |
| PIK3R3 | SRC | YES1 | PIK3R1 |
| VAV2 | LYN | SHD | VAV2 |
| PIK3R1 | YES1 | PIK3R1 | SYK |
| VAV3 | SHC1 | GRB2 | PIK3R3 |
| SHB | RASA1 | LCK | SLA2 |
| TEC | SYK | PTK6 | VAV3 |
The 15 proteins considered in the first column are those expressed in HEK 293 cells according to Geiger et al. (36) and that also have a measured KD for at least one EGFR phosphotyrosine, as determined in the study of Hause et al. (5). Bold text is used to indicate proteins detected to bind EGFR (or more precisely, to coimmunoprecipitate with EGFR) in the study of Tong et al. (39). It should be noted that copy numbers determined by Tong et al. (39) were not used in our analysis. In each column, EGFR binding partners are ranked according to the indicated importance metric: the simulation, copy number (#), KD or ratio (#/KD) metric. The most important protein is listed at the top of each column. Except for the third column, rankings are based on the protein copy measurements of Geiger et al. (36). The rankings of the third column are based on the KD measurements of Hause et al. (5): the ranking of a protein is determined by the minimum KD among all interactions of the protein with phosphotyrosines in EGFR. The protein with highest affinity is at the top.