Table 2.
Measured 13C chemical shifts and corresponding secondary structural assignments
| Assignment | Structure | ||
|---|---|---|---|
| Untreated sample | |||
| DP | CP | ||
| 11.9 ± 0.5 | 11.5 ± 0.5 | Cδ-Ile | RC |
| — | 17.9 | Cβ-Ala, Cγ-Val | β-sheet/turn or RC, RC |
| — | 17.2 | Cβ-Ala | α-helix or β-sheet/turn or RC |
| 18.3 ± 0.9 | 18.0 ± 0.5 | Cβ-Ala, Cγ-Val | β-sheet/turn or RC, RC |
| 19.0 ± 0.5 | 19.0 ± 0.5 | Cβ-Ala, Cγ-Val | β-sheet/turn, RC |
| 20.1 ± 0.5 | 20.1 ± 0.5 | Cβ-Ala, Cγ-Val | β-sheet/turn, RC |
| 22.5 ± 0.5 | 22.5 | — | — |
| 24.0 ± 0.5 | 23.7 | — | — |
| 25.7 ± 0.5 | 25.7 | — | — |
| 31.4 ± 0.9 | 31.4 ± 0.5 | Cβ-Val, Cβ-Pro | —, — |
| 37.8 ± 0.9 | 37.8 ± 0.5 | Cβ-Phe, Cβ-Ile | —, β-sheet/turn or RC |
| 41.0 ± 0.5 | — | Cβ-Phe, Cβ-Leu | β-sheet/turn, — |
| 44.2 ± 0.9 | 44.1 ± 0.5 | Cα-Gly, Cβ-Leu | —, β-sheet/turn |
| 49.2 ± 0.9 | — | Cα-Ala | β-sheet/turn or RC |
| 51.3 ± 0.7 | 51.6 ± 0.5 | Cα-Ala, Cα-Leu | β-sheet/turn or RC, β-sheet/turn |
| 53.8 ± 0.9 | — | Cα-Ala, Cα-Phe | α-helix or RC, RC or β-sheet/turn |
| 56.2 | — | Cα-Phe, Cα-Leu | β-sheet/turn or RC, α-helix |
| 58.0 ± 0.3 | — | Cα-Val, Cα-Phe | β-sheet/turn or RC, α-helix |
| 60.9 ± 0.5 | 60.8 ± 0.5 | Cα-Val, Cα-Pro | RC or β-sheet/turn, RC or β-sheet/turn |
| 61.9 ± 0.5 | 62.3 ± 0.5 | Cα-Val, Cα-Pro | RC or β-sheet/turn, — |
| 68.7 ± 0.5 | Cβ-Thr | β-sheet/turn or RC | |
| 71.2 ± 0.5 | Cβ-Thr | β-sheet/turn | |
| Glucose-treated sample | |||
| DP | CP | ||
| 11.9 ± 0.4 | 11.9 ± 0.5 | Cδ-Ile | RC |
| 16.9 ± 0.7 | 16.9 ± 0.7 | Cβ-Ala | RC or α-helix |
| 19.3 ± 0.4 | — | Cβ-Ala, Cγ-Val | β-sheet/turn, RC |
| 20.1 ± 0.4 | 20.1 ± 0.7 | Cβ-Ala, Cγ-Val | β-sheet/turn, RC |
| 22.5 ± 0.5 | 22.5 ± 0.4 | — | — |
| 24.0 ± 0.7 | 24.0 ± 0.7 | — | — |
| 25.7 ± 0.7 | 25.7 ± 0.9 | — | — |
| 31.1 ± 1.4 | 31.1 ± 1.4 | Cβ-Val, Cβ-Pro | —, — |
| 37.4 | 37.8 | Cβ-Phe, Cβ-Ile | —, β-sheet/turn or RC |
| 41.0 | 41.0 | Cβ-Phe, Cβ-Leu | β-sheet/turn, — |
| 42.4 | — | Cα-Gly, Cβ-Leu | —, β-sheet/turn or RC |
| 43.8 | 43.8 | Cα-Gly, Cβ-Leu | —, β-sheet/turn |
| 48.4 | 49.2 ± 0.7 | Cα-Ala | β-sheet/turn or RC |
| 50.9 | — | Cα-Ala | β-sheet/turn or RC |
| 53.8 ± 0.5 | Cα-Ala, Cα-Phe | RC or α-helix, β-sheet/turn or RC | |
| — | 58.0 | Cα-Val, Cα-Phe | β-sheet/turn or RC, α-helix |
Data are based on values in Table 1 for hydrated untreated and glucose-treated porcine aortic elastin under DP and CP conditions. RC, random coil; —, no signal was observed or no assignment could be made.