Spectral images of representative CHO cells expressing the cytoplasmic constructs N5D or ADAA. (Upper panels) The samples were irradiated by two-photon lasers at 800 and 1020 nm for Cerulean and Venus, respectively, to obtain the reconstructed images at the emission wavelengths shown. (Lower panel plots, cyan and yellow lines) Elementary emission spectra of Cerulean and Venus, respectively, averaged over nonzero pixels (region indicated by the red circle in each image).