Figure 8.

Comparison of Cyc1 and Cyc10 chromatograms from 5'-rMP reaction on DNA Pac PA200 column obtained using a fluorescence detector (λ_ex = 365 nm and λ_em = 425 nm). Polymerization takes place immediately after one cycle of DH-RH at pH 2, as is evident from the multiple peaks obtained (blue trace). Greater polymerization resulting in longer oligomers seems to occur when 5'-rMP monomers are subjected to 10 DH-RH; such cycles as indicated in the black trace. The green arrow denotes the retention time of 5'-AMP on the same gradient for comparison.