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. 2015 Jan 5;5(1):85–101. doi: 10.3390/life5010085

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Examination of posttranslational modifications of archaellins and pilins in various mutant backgrounds. (A) Western blot detection of archaellin FlaB2 in various M. maripaludis mutant strains. Whole cell lysates were separated by SDS-PAGE (15% gel), transferred to Immobilon membrane and the blot developed with antibodies to FlaB2. (B) Western blot detection of EpdE in various M. maripaludis mutant strains expressing a plasmid borne C-terminal FLAG-tagged version of EpdE. (C) Western blot detection of EpdD in various M. maripaludis mutant strains expressing a plasmid borne C-terminal FLAG-tagged version of EpdD. For panels (B) and (C), whole cell lysates were separated by SDS-PAGE (17.5% gel), transferred to Immobilon membrane and developed with antibodies to the FLAG-tag.