Skip to main content
. 2015 Jan 5;5(1):85–101. doi: 10.3390/life5010085

Table 2.

Primers used in this study *.

Primers Primer Sequence 5’ to 3’ Restriction site
1685+3_sdm_For Nsi1 CCAATGCATGAAATTTTTAGAAAAACTAACATCAAAAAAAGGTC
AAATAGGAATGGAACTCGG *
Nsi1
1685+1+3_sdm_For Nsi1 CCAATGCATGAAATTTTTAGAAAAACTAACATCAAAAAAAGGTG
CAATAGGAATGGAACTCGG *
Nsi1
1685_Mlu_Rev AGCACGCGTTTAATCCGTAATATTTGACATTTGTGAGG Mlu1
1685_Nsi1_For CCAATGCATGAAATTTTTAGAAAAACTAACATC Nsi1
1685_histag_Mlu_Rev CGCGACGCGTTTAGTGATGGTGGTGATGGTGATCCGTAATA
TTTGACATTTGTGAGG
Mlu1
1685_FLAG_Mlu_Rev CGACGCGTTTATTTGTCATCGTCATCTTTGTAATCATCCGTA
ATATTTGACATTTGTGAG
Mlu1
1685_For_exp GGAATTCCATATGATGAAATTTTTAGAAAAACTAACATC NdeI
1685-Rev_exp CCGCTCGAGATCCGTAATATTTGACATTTGTGAG XhoI
1283_Nsi1_For CCAATGCATGTCTGTTGCTTTAAAGAAGTTTTTTTCGAAACG Nsi1
1283_FLAG_Mlu_Rev GCTACGCGTTTATTTGTCATCGTCATCTTTGTAATCACTAACTT
CACTTAAAGCATCTAT
Mlu1
FlaK_seq_For AATATCTGGCGGATACAGG -
FlaK_seq_Rev TTCAAAGCCAATAGATACTGC -
EppA_seq_For CTGGAGCTGTATGAAATGCAAC -
EppA_seq_Rev GGATGACCTGGGATAATGCAGG -
AglB_seq_For CATAACCATATTTGTAATTAAC -
AglB_seq_Rev CTCAATAGCCATAAAATCACC -

* Restriction enzyme sites added to primers are underlined. SDM changes are shown in bold italics.