Table 2.
Primers | Primer Sequence 5’ to 3’ | Restriction site |
---|---|---|
1685+3_sdm_For Nsi1 | CCAATGCATGAAATTTTTAGAAAAACTAACATCAAAAAAAGGTC AAATAGGAATGGAACTCGG * |
Nsi1 |
1685+1+3_sdm_For Nsi1 | CCAATGCATGAAATTTTTAGAAAAACTAACATCAAAAAAAGGTG CAATAGGAATGGAACTCGG * |
Nsi1 |
1685_Mlu_Rev | AGCACGCGTTTAATCCGTAATATTTGACATTTGTGAGG | Mlu1 |
1685_Nsi1_For | CCAATGCATGAAATTTTTAGAAAAACTAACATC | Nsi1 |
1685_histag_Mlu_Rev | CGCGACGCGTTTAGTGATGGTGGTGATGGTGATCCGTAATA TTTGACATTTGTGAGG |
Mlu1 |
1685_FLAG_Mlu_Rev | CGACGCGTTTATTTGTCATCGTCATCTTTGTAATCATCCGTA ATATTTGACATTTGTGAG |
Mlu1 |
1685_For_exp | GGAATTCCATATGATGAAATTTTTAGAAAAACTAACATC | NdeI |
1685-Rev_exp | CCGCTCGAGATCCGTAATATTTGACATTTGTGAG | XhoI |
1283_Nsi1_For | CCAATGCATGTCTGTTGCTTTAAAGAAGTTTTTTTCGAAACG | Nsi1 |
1283_FLAG_Mlu_Rev | GCTACGCGTTTATTTGTCATCGTCATCTTTGTAATCACTAACTT CACTTAAAGCATCTAT |
Mlu1 |
FlaK_seq_For | AATATCTGGCGGATACAGG | - |
FlaK_seq_Rev | TTCAAAGCCAATAGATACTGC | - |
EppA_seq_For | CTGGAGCTGTATGAAATGCAAC | - |
EppA_seq_Rev | GGATGACCTGGGATAATGCAGG | - |
AglB_seq_For | CATAACCATATTTGTAATTAAC | - |
AglB_seq_Rev | CTCAATAGCCATAAAATCACC | - |
* Restriction enzyme sites added to primers are underlined. SDM changes are shown in bold italics.