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Relationship between TrpRS Urzyme and connecting peptide 1 (CP1). The alpha carbons to which the N and C termini of the insertion attach are separated by almost exactly the distance of a peptide bond (blue dots). This enables its removal by protein engineering, which was accomplished using the Rosetta design program [15,21]. The dashed arrow indicates where the anticodon-binding domain attaches (Adapted from [15]).
