Figure 2.
Arrangement of gvp genes in p-vac of Hbt. salinarum PHH1 (a) and a comparison of the intergenic regions separating PD and PA (b). (a) The arrows depicting genes are colored as follows: dark green, encoding structural proteins of the A-J-M family and GvpC; light green, encoding accessory Gvp; red, encoding regulator proteins. Black arrows mark the start sites of transcription. (b) Comparison of the intergenic regions separating PA and PD in p-, mc- and c-vac. A 22-nt insertion occurs in c-vac adjacent to BRED. The 20-nt sequence required for GvpE-mediated activation is underlined (8-nt elements separated by 4 nt of unimportant sequences) in the case of UASA and marked by an arrow. Similar activation elements are found with UASD, pointing in the opposite direction. The TATA-box and BRE sequences (italics) are shaded in grey.