Table 1.
Substrate preferences of prokaryotic Argonautes proteins in vitro and in vivo. n.d. = not determined; inactive = no catalytic tetrad present; ( ) = significantly reduced affinity; # = determined in vitro; * = determined in vivo; { } unpublished.
| Argonaute Variant | Guide Strand Bound | Preference/Enrichment For 1st Guide Nucleotide | Target Strands Bound | Target Strands Cleaved | Ref. |
|---|---|---|---|---|---|
| Archaea | |||||
| M. jannaschii Ago | DNA #, (RNA) # | {G} # | DNA #, RNA # | DNA # | [10] |
| A. fulgidus Ago | DNA #, (RNA) # | n.d. | DNA #, RNA # | n.d. | [19] |
| Bacteria | |||||
| A. aeolicus Ago | DNA *, (RNA) * | n.d. | RNA #, DNA # | n.d. | [69] |
| R. sphaeroides Ago | RNA *, DNA * | U * | RNA *, plasmid DNA# | inactive | [8] |
| T. thermophilus Ago | DNA *,#, (RNA) # | C * | DNA *,#, RNA #, plasmid DNA *,# | DNA #, RNA #, plasmid DNA *,# | [9,14] |