. 2015 Mar 12;5(1):861–871. doi: 10.3390/life5010861

Table 1.

Activity of MtACK wild type and variants with each nucleotide triphosphate (NTP).

Enzyme	Specific Activity ^a (mol·min⁻¹·mg⁻¹)	Percent Activity Relative to That Observed with 10 mM ATP ^b
Enzyme	Specific Activity ^a (mol·min⁻¹·mg⁻¹)	ATP	CTP	GTP	TTP	UTP	ITP
Wild type	883 ± 13	100.0 ± 1.4	50.0 ± 0.4	85.2 ± 1.9	64.5 ± 0.7	54.8 ± 0.5	80.0 ± 0.5
Asn211Ala	145 ± 1.6	100.0 ± 1.1	56.4 ± 0.6	65.5 ± 1.1	78.6 ± 2.5	43.0 ± 0.3	80.0 ± 1.1
Asn211Ser	4.4 ± 0.04	100.0 ± 1.0	56.9 ± 0.6	65.7 ± 0.5	73.9 ± 0.4	43.7 ± 0.2	83.8 ± 0.7
Asn211Thr	20 ± 0.1	100.0 ± 0.6	55.7 ± 0.2	62.1 ± 0.9	95.7 ± 0.5	28.4 ± 0.3	75.4 ± 1.4
Gly239Ala	61 ± 1.7	100.0 ± 2.8	90.9 ± 1.0	64.3 ± 0.6	142.0 ± 0.9	60.2 ± 1.5	51.8 ± 0.5
Gly239Ser	4.6 ± 0.06	100.0 ± 1.2	93.7 ± 1.0	72.1 ± 0.7	163.4 ± 1.6	72.8 ± 0.7	56.2 ± 0.4
Gly331Ala	58 ± 1.0	100.0 ± 1.6	9.7 ± 0.5	49.0 ± 0.4	8.0 ± 0.3	4.4 ± 0.3	62.5 ± 0.1
Gly331Gln	7.5 ± 0.05	100.0 ± 0.6	11.8 ± 0.9	46.6 ± 0.5	8.1 ± 0.6	5.1 ± 0.4	69.0 ± 0.5

Notes: ^a Specific activity determined with 10 mM ATP; ^b Activities were determined at 10 mM final NTP concentration.