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Bright field (left) and fluorescence confocal images (center, right) of lipid vesicles prepared by the film hydration method (M1) starting from a FITC-dextran solution. Vesicles were made of POPC and 0.05 mol% DOPE-lissamine-rhodamine in order to be red-fluorescent (central image). Note that despite the abundant presence of vesicles, only few of them have encapsulated a high amount of green-fluorescent solute (right image) so to appear more fluorescent than the background.
