Table 1. Pearson correlation coefficients between MS protein abundance and ribo-seq coverage.
MS protein identifications were performed with an FDR of 1% and protein abundances were calculated as emPAI and NSAF values. The correlation coefficients were computed for each of the following transcript filtering settings: i) all dbTIS transcripts without additional thresholds, ii) only transcripts with a validated MS identification (i.e. transcripts with a spectral count value > 2), iii) only dbTIS transcripts with a total RPF count ≥ 200 and iv) only dbTIS transcripts with both a validated MS/MS-based identification and an RPF count ≥ 200.
| i | ii | iii | iv | |
|---|---|---|---|---|
| 1% FDR | ||||
| emPAI | 0.488 | 0.498 | 0.483 | 0.518 |
| NSAF | 0.608 | 0.642 | 0.634 | 0.664 |