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. Author manuscript; available in PMC: 2015 Apr 9.
Published in final edited form as: Mol Microbiol. 2011 Nov 7;82(5):1150–1163. doi: 10.1111/j.1365-2958.2011.07875.x

Fig. 9.

Fig. 9

Complementation of the Δagl3 deletion mutant. Equivalent amounts of the S-layer protein SlaA isolated from S. acidocaldarius MW001 (lane 1), Δagl3 (lane 2), Δagl3 complemented with pSVA1266 (lane 3), and Δagl3 with control plasmid pSVA1450 (lane 4) were separated by 8% SDS-PAGE and either (A) Coomassie blue-stained or (B) stained for carbohydrates using Emerald 300 (B).