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. 2015 Mar 29;15:33. doi: 10.1186/s12935-015-0186-0

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© Shi et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Apigenin on the protein levels of proapoptotic proteins, antiapoptotic proteins, and the activation of the caspases cascade in T-24 cells. Cell were treated with various concentrations of apigenin for 24 h and 30 μg/ml apigenin for various periods of time (0, 1, 3, 6, and 12 h), and total cell lysates were prepared to detect (A) the protein levels of Bcl-xL, Bcl-2, Mcl-1, Bax, Bad, and Bak. (B) cleavage of caspase-3, caspase-7, caspase-9, and PARP by Western blotting analysis. The results were represented by using an ECL system and β-actin was the internal control. The folds were compared with control. The results were obtained from at least three independent measurements.