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. 2015 Mar 29;15:33. doi: 10.1186/s12935-015-0186-0

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© Shi et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The effect of apigenin on the intracellular levels of ROS and GSH in T-24 cells. Cells were treated with 30 μg/ml apigenin for various periods (0, 1, 2, 3, 6, and 12 h), and then were analysed for (A) the intracellular ROS production (B) the intracellular GSH content by spectrofluorophotometer as described in “Materials and Methods” section. The results represented the average of three independent experiments ± S.D. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the 0 h-treated time.