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. 2014 Nov 18;6(1):27. doi: 10.1186/1868-7083-6-27

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© Zarfeshani et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression of the genes involved in glucose and lipid sensing after leucine supplementation of HepG2 cells. (A) Real-time PCR (qPCR) and immunoblotting analysis of expression levels of genes involved in gluconeogenesis, glucose uptake and lipogenesis. (B) qPCR and immunoblotting analysis of specific upstream genes involved in glucose/lipid sensing. Western blotting was performed using cell lysates supplemented with leucine for 48 h. Values are presented as mean ± SEM. Statistical significance relative to untreated control, *P <0.05 (n =3).