Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Nov 18;6(1):27. doi: 10.1186/1868-7083-6-27

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Zarfeshani et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Discovery and validation of miRNA expression after leucine supplementation. (A) Cluster analysis of miRNAs available on Affymetrix miRNA chips. The red color shows relatively abundant expression of the same miRNA when compared to controls, whereas the blue color indicates a low expression relative to other samples. Cluster analysis was performed with Gene Spring GX. (B) Validation of specific miRNA species exhibiting significant difference from the control group, using real-time PCR (qPCR). Finally, differential expression of specific target genes measured after inhibition of miR-143 (C), miR-92b*(D) and miR-335 (E) using selective siRNAs. Values are presented as the mean ± SEM. Statistical significance relative to untreated control, *P <0.05, **P <0.01 (n =3).