Table 1.

A population study of the effect of carbon black nanomaterial on erythrocyte shape

	Sample size (number of frames)	Test			Control			Difference between test and control
Sample	Test/Control	D: % discocytes (SD)	E: % echinocytes (SD)	S: % stomatocytes (SD)	D: % discocytes (SD)	E: % echinocytes (SD)	S: % stomatocytes (SD)	ΔD (%) (p, P)	ΔE (%) (p, P)	ΔS (%) (p, P)
1 hour	56/54	99 (2)	1 (1)	0	90 (8)	9 (7)	1 (5)	9 (<10-6, 1)	-8 (<10-6, 1)	NA
3 hours	50/53	90 (18)	9 (18)	0	84 (11)	16 (11)	0	6 (0.05, 0.62)	-6 (0.04, 0.75)	NA
24 hours	49/51	79 (9)	21 (9)	0	62 (9)	38 (10)	0	17 (<10-6, 1)	-17 (<10-6, 1)	NA

A population study of the effect of carbon black nanomaterial on erythrocyte shape. The average values of the portion of discocytes (D), echinocytes (E) and stomatocytes (S) are given after 1 hour, 3 hours and 24 hours of incubation with the carbon black nanomaterial (test sample) and with citrated and phosphate buffered saline (control sample). SD: standard deviation. p: statistical significance of the difference between the average values of the test and control, calculated by the t-test. P: statistical power. NA: non applicable as there were les than 1% of stomatocytes in all samples. Altogether 10712 cells were included in the analysis.