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. 2015 Apr 3;19(1):138. doi: 10.1186/s13054-015-0878-9

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© Villar et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Western blot analysis of tryptase. The upper panel is a representative gel for each experimental group. The lower panel represents the relative optical density of tryptase normalized against β-actin. C: non-septic, anesthetized, non-ventilated, spontaneous breathing; S: septic, anesthetized, non-ventilated, spontaneous breathing; SLVT + PEEP: septic, ventilated with low tidal volume plus positive end-expiratory pressure (PEEP) of 10 cmH₂O; SHVT: septic, ventilated with high tidal volume. Six animals were quantified in each group. ^# P < 0.01 vs. S; ^¶ P <0.001 vs. S.