Table 1.
Control | Ecf | ETEC | EPEC | Sampling time | |
---|---|---|---|---|---|
TEER (Ohm∗cm2) | 4760 ± 1283 | 4744 ± 1187 | 3021 ± 2341 | 4590 ± 1263 | 4 h |
Mannitol flux (nmol∗cm−2∗h−1) | 4.4 ± 3.2 | 3.9 ± 3.7 | 6.5 ± 5.4 | 15.2 ± 13.8 | |
n TEER∣n Mannitol | 14∣6 | 15∣7 | 14∣9 | 12∣6 | |
TEER (Ohm∗cm2) | 3518 ± 1397a | 3692 ± 1177a | 2690 ± 2428ab | 1115 ± 1018b | 8 h |
Mannitol flux (nmol∗cm−2∗h−1) | 2.1 ± 2.6a | 3.4 ± 2.0a | 11 ± 12.2ab | 26.9 ± 25.1b | 8 h |
n TEER∣n Mannitol | 13∣8 | 14∣7 | 13∣4 | 11∣6 |
Bacterial strains: IPEC-J2 cells were incubated with E. faecium (Ecf), ETEC, or EPEC for 4 h and 8 h (mean ± standard deviation, variance analysis, post hoc Scheffe test, P ≤ 0.05, and different letters indicate significant differences between incubation groups); n = cell culture inserts.