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. 2015 Mar 29;15:199. doi: 10.1186/s12885-015-1217-x

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© Martínez-Aguilar et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Determination of reference proteins using the geNorm algorithm. a) Average expression stability values of candidate reference proteins; b) Determination of the optimal number of reference proteins for normalisation. Pairwise variations Vn/n + 1 are calculated between two sequential normalisation factors and stepwise inclusion of the most stable remaining control protein.