Table 2.
Targeted S100 peptide sequences (region 7-14 kDa)
| Q1 | Q3 | Rt | Protein.peptide.transition ion.isotopologue | CE | CEXP |
|---|---|---|---|---|---|
| 846.45 | 778.41 | 31.5 | S100A1.ELLQTELSGFLDAQK.y7.light | 39.3 | 13 |
| 846.45 | 865.44 | 31.5 | S100A1.ELLQTELSGFLDAQK.y8.light | 39.3 | 13 |
| 846.45 | 1208.62 | 31.5 | S100A1.ELLQTELSGFLDAQK.y11.light | 39.3 | 13 |
| 503.27 | 763.40 | 23.5 | S100A2.ELPSFVGEK. + 2y7.light | 22.7 | 12 |
| 503.27 | 666.35 | 23.5 | S100A2.ELPSFVGEK. + 2y6.light | 30.7 | 11 |
| 503.27 | 579.31 | 23.5 | S100A2.ELPSFVGEK. + 2y5.light | 30.7 | 9 |
| 507.27 | 771.41 | 23.5 | S100A2.ELPSFVGEK. + 2y7.heavy | 22.7 | 12 |
| 507.27 | 674.36 | 23.5 | S100A2.ELPSFVGEK. + 2y6.heavy | 30.7 | 11 |
| 507.27 | 587.33 | 23.5 | S100A2.ELPSFVGEK. + 2y5.heavy | 30.7 | 9 |
| 445.75 | 648.37 | 25.5 | S100A4.ELPSFLGK. + 2y6.light | 21.5 | 19 |
| 445.75 | 551.32 | 25.5 | S100A4.ELPSFLGK. + 2y5.light | 25.5 | 19 |
| 445.75 | 464.29 | 25.5 | S100A4.ELPSFLGK. + 2y4.light | 25.5 | 16 |
| 449.76 | 656.39 | 25.5 | S100A4.ELPSFLGK. + 2y6.heavy | 21.5 | 19 |
| 449.76 | 559.33 | 25.5 | S100A4.ELPSFLGK. + 2y5.heavy | 25.5 | 19 |
| 449.76 | 472.30 | 25.5 | S100A4.ELPSFLGK. + 2y4.heavy | 25.5 | 16 |
| 455.22 | 693.36 | 16.0 | S100A4.TDEAAFQK. + 2y6.light | 21.2 | 20 |
| 455.22 | 564.31 | 16.0 | S100A4.TDEAAFQK. + 2y5.light | 21.2 | 20 |
| 455.22 | 493.28 | 16.0 | S100A4.TDEAAFQK. + 2y4.light | 21.2 | 14 |
| 459.23 | 701.37 | 16.0 | S100A4.TDEAAFQK. + 2y6.heavy | 21.2 | 20 |
| 459.23 | 572.33 | 16.0 | S100A4.TDEAAFQK. + 2y5.heavy | 21.2 | 20 |
| 459.23 | 501.29 | 16.0 | S100A4.TDEAAFQK. + 2y4.heavy | 21.2 | 14 |
| 374.22 | 618.38 | 18.5 | S100A6.ELTIGSK. + 2y6.light | 20.3 | 13 |
| 374.22 | 505.30 | 18.5 | S100A6.ELTIGSK. + 2y5.light | 20.3 | 13 |
| 374.22 | 404.25 | 18.5 | S100A6.ELTIGSK. + 2y4.light | 20.3 | 13 |
| 378.22 | 626.40 | 18.5 | S100A6.ELTIGSK. + 2y6.heavy | 20.3 | 13 |
| 378.22 | 513.31 | 18.5 | S100A6.ELTIGSK. + 2y5.heavy | 20.3 | 13 |
| 378.22 | 412.26 | 18.5 | S100A6.ELTIGSK. + 2y4.heavy | 20.3 | 13 |
| 458.25 | 802.41 | 17.5 | S100A6.LQDAEIAR. + 2y7.light | 25.4 | 13 |
| 458.25 | 674.35 | 17.5 | S100A6.LQDAEIAR. + 2y6.light | 23.4 | 20 |
| 458.25 | 559.32 | 17.5 | S100A6.LQDAEIAR. + 2y5.light | 31.4 | 17 |
| 463.25 | 812.41 | 17.5 | S100A6.LQDAEIAR. + 2y7.heavy | 25.4 | 13 |
| 463.25 | 684.36 | 17.5 | S100A6.LQDAEIAR. + 2y6.heavy | 23.4 | 20 |
| 463.25 | 569.33 | 17.5 | S100A6.LQDAEIAR. + 2y5.heavy | 31.4 | 17 |
| 636.85 | 774.41 | 24.5 | s100A8.ALNSIIDVYHK.y6.light | 31.8 | 13 |
| 636.85 | 974.53 | 24.5 | s100A8.ALNSIIDVYHK.y8.light | 31.8 | 13 |
| 636.85 | 887.50 | 24.5 | s100A8.ALNSIIDVYHK.y7.light | 31.8 | 13 |
| 640.86 | 782.42 | 24.5 | S100A8.ALNSIIDVYHK.y6.heavy | 31.9 | 13 |
| 640.86 | 982.54 | 24.5 | S100A8.ALNSIIDVYHK.y8.heavy | 31.9 | 13 |
| 640.86 | 895.51 | 24.5 | S100A8.ALNSIIDVYHK.y7.heavy | 31.9 | 13 |
| 602.98 | 790.42 | 30.0 | S100A9.NIETIINTFHQYSVK.y13.light | 30.4 | 13 |
| 602.98 | 725.89 | 30.0 | S100A9.NIETIINTFHQYSVK.y12.light | 30.4 | 13 |
| 602.98 | 618.83 | 30.0 | S100A9.NIETIINTFHQYSVK.y10.light | 30.4 | 13 |
| 605.65 | 794.43 | 30.0 | S100A9.NIETIINTFHQYSVK.y13.heavy | 30.5 | 13 |
| 605.65 | 729.90 | 30.0 | S100A9.NIETIINTFHQYSVK.y12.heavy | 30.5 | 13 |
| 605.65 | 622.84 | 30.0 | S100A9.NIETIINTFHQYSVK.y10.heavy | 30.5 | 13 |
| 379.21 | 642.38 | 18.0 | S100A10.DPLAVDK. + 2y6.light | 22.5 | 19 |
| 379.21 | 545.33 | 18.0 | S100A10.DPLAVDK. + 2y5.light | 20.5 | 17 |
| 379.21 | 432.25 | 18.0 | S100A10.DPLAVDK. + 2y4.light | 20.5 | 17 |
| 383.22 | 650.40 | 18.0 | S100A10.DPLAVDK. + 2y6.heavy | 22.5 | 19 |
| 383.22 | 553.34 | 18.0 | S100A10.DPLAVDK. + 2y5.heavy | 20.5 | 17 |
| 383.22 | 440.26 | 18.0 | S100A10.DPLAVDK. + 2y4.heavy | 20.5 | 17 |
| 604.80 | 932.48 | 26.0 | S100A10.EFPGFLENQK. + 2y8.light | 26.6 | 13 |
| 604.80 | 835.43 | 26.0 | S100A10.EFPGFLENQK. + 2y7.light | 34.6 | 13 |
| 604.80 | 466.75 | 26.0 | S100A10.EFPGFLENQK. + 2y8 + 2.light | 26.6 | 13 |
| 608.81 | 940.50 | 26.0 | S100A10.EFPGFLENQK. + 2y8.heavy | 26.6 | 13 |
| 608.81 | 843.45 | 26.0 | S100A10.EFPGFLENQK. + 2y7.heavy | 34.6 | 13 |
| 608.81 | 470.75 | 26.0 | S100A10.EFPGFLENQK. + 2y8 + 2.heavy | 26.6 | 13 |
| 386.20 | 559.32 | 18.0 | S100A11.DPGVLDR. + 2y5.light | 24.2 | 18 |
| 386.20 | 502.30 | 18.0 | S100A11.DPGVLDR. + 2y4.light | 24.2 | 18 |
| 386.20 | 403.23 | 18.0 | S100A11.DPGVLDR. + 2y3.light | 20.2 | 18 |
| 391.21 | 569.33 | 18.0 | S100A11.DPGVLDR. + 2y5.heavy | 24.2 | 18 |
| 391.21 | 512.31 | 18.0 | S100A11.DPGVLDR. + 2y4.heavy | 24.2 | 18 |
| 391.21 | 413.24 | 18.0 | S100A11.DPGVLDR. + 2y3.heavy | 20.2 | 18 |
| 530.75 | 888.45 | 20.0 | S100A11.DGYNYTLSK. + 2y7.light | 24.0 | 14 |
| 530.75 | 725.38 | 20.0 | S100A11.DGYNYTLSK. + 2y6.light | 24.0 | 12 |
| 530.75 | 611.34 | 20.0 | S100A11.DGYNYTLSK. + 2y5.light | 24.0 | 18 |
| 534.76 | 896.46 | 20.0 | S100A11.DGYNYTLSK. + 2y7.heavy | 24.0 | 14 |
| 534.76 | 733.40 | 20.0 | S100A11.DGYNYTLSK. + 2y6.heavy | 24.0 | 12 |
| 534.76 | 619.35 | 20.0 | S100A11.DGYNYTLSK. + 2y5.heavy | 24.0 | 18 |
| 519.76 | 836.45 | 22.5 | S100A13.DSLSVNEFK. + 2y7.light | 23.6 | 13 |
| 519.76 | 723.37 | 22.5 | S100A13.DSLSVNEFK. + 2y6.light | 23.6 | 20 |
| 519.76 | 636.34 | 22.5 | S100A13.DSLSVNEFK. + 2y5.light | 23.6 | 20 |
| 523.77 | 844.47 | 22.5 | S100A13.DSLSVNEFK. + 2y7.heavy | 23.6 | 13 |
| 523.77 | 731.38 | 22.5 | S100A13.DSLSVNEFK. + 2y6.heavy | 23.6 | 20 |
| 523.77 | 644.35 | 22.5 | S100A13.DSLSVNEFK. + 2y5.heavy | 23.6 | 20 |
| 372.24 | 630.38 | 20.5 | S100A13.LIGELAK. + 2y6.light | 19.5 | 19 |
| 372.24 | 517.30 | 20.5 | S100A13.LIGELAK. + 2y5.light | 17.5 | 15 |
| 372.24 | 460.28 | 20.5 | S100A13.LIGELAK. + 2y4.light | 25.5 | 16 |
| 376.24 | 638.40 | 20.5 | S100A13.LIGELAK. + 2y6.heavy | 19.5 | 19 |
| 376.24 | 525.31 | 20.5 | S100A13.LIGELAK. + 2y5.heavy | 17.5 | 15 |
| 376.24 | 468.29 | 20.5 | S100A13.LIGELAK. + 2y4.heavy | 25.5 | 16 |
| 624.31 | 1047.50 | 20.0 | S100A13.SLDVNQDSELK. + 2y9.light | 31.3 | 16 |
| 624.31 | 932.47 | 20.0 | S100A13.SLDVNQDSELK. + 2y8.light | 31.3 | 16 |
| 624.31 | 833.40 | 20.0 | S100A13.SLDVNQDSELK. + 2y7.light | 31.3 | 14 |
| 628.32 | 1055.51 | 20.0 | S100A13.SLDVNQDSELK. + 2y9.heavy | 31.3 | 16 |
| 628.32 | 940.48 | 20.0 | S100A13.SLDVNQDSELK. + 2y8.heavy | 31.3 | 16 |
| 628.32 | 841.41 | 20.0 | S100A13.SLDVNQDSELK. + 2y7.heavy | 31.3 | 14 |
| 625.82 | 1016.54 | 31.5 | S100A14.SFWELIGEAAK. + 2y9.light | 29.0 | 17 |
| 625.82 | 830.46 | 31.5 | S100A14.SFWELIGEAAK. + 2y8.light | 29.0 | 13 |
| 625.82 | 701.42 | 31.5 | S100A14.SFWELIGEAAK. + 2y7.light | 32.0 | 12 |
| 629.83 | 1024.56 | 31.5 | S100A14.SFWELIGEAAK. + 2y9.heavy | 29.0 | 17 |
| 629.83 | 838.48 | 31.5 | S100A14.SFWELIGEAAK. + 2y8.heavy | 29.0 | 13 |
| 629.83 | 709.43 | 31.5 | S100A14.SFWELIGEAAK. + 2y7.heavy | 32.0 | 12 |
| 647.87 | 912.48 | 31.0 | S100A16.AVIVLVENFYK. + 2y7.light | 28.0 | 14 |
| 647.87 | 799.40 | 31.0 | S100A16.AVIVLVENFYK. + 2y6.light | 24.0 | 13 |
| 647.87 | 700.33 | 31.0 | S100A16.AVIVLVENFYK. + 2y5.light | 26.0 | 12 |
| 651.88 | 920.50 | 31.0 | S100A16.AVIVLVENFYK. + 2y7.heavy | 28.0 | 14 |
| 651.88 | 807.41 | 31.0 | S100A16.AVIVLVENFYK. + 2y6.heavy | 24.0 | 13 |
| 651.88 | 708.34 | 31.0 | S100A16.AVIVLVENFYK. + 2y5.heavy | 26.0 | 12 |
Rt: retention time (min), CE: collision energy; CEXP: cell exit potential.