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GFP expression in cultered RGCs. (A) GFP is expressed in somata and axons of dissociated RGCs at 4 days in culture. (A’) Merged view of bright-field and fluorescent image. Scale bar = 100 µm. (B) Quantification of neurite length per RGC in retinal cultures of GAP43:GFP zebrafish. Addition of 2 ng/ml CNTF significantly induced neurite growth. Data represent means ± SEM of 6 wells from two independent experiments. Treatment effects (asterisks): p < 0.001.
