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. 2015 Apr 9;9:118. doi: 10.3389/fncel.2015.00118

Figure 4.

Figure 4

GFP expression in the optic nerve. (A) Transverse section of the optic nerve reveals GFP expression in a discrete bundle in the periphery (arrow). The white line indicates the outline of the nerve. (B) At 7 days after injury, regenerating RGC axons across the whole optic nerve transverse section are GFP-positive. Scale bar = 25 µm. (C) Longitudinal view of a cleared, naïve wholemount optic nerve (maximum intensity projection of a confocal stack). In addition to the labeled peripheral axon bundle (arrow), single GFP-positive axons are visible throughout the optic nerve (arrowheads). Scale bar = 200 µm. (C’) Higher magnification of the boxed area in (C) using one Z-section of a cleared, naïve wholemount optic nerve reveals single axons within the peripheral bundle (arrows). Scale bar = 50 µm.