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. 2015 Apr 9;9:120. doi: 10.3389/fncel.2015.00120

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Copyright © 2015 Kaczor, Rakus and Mozrzymas.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Supplementation of neuronal culture (NC) with astrocytes increases the number of synaptic puncta. (A,B), typical examples of staining for synaptic puncta with antibodies against GAD65 (red) in NC (A) and ANCC (B). Green color reflects tubulin β3 staining in all graphs. (C) Statistics for the number of GAD65 puncta per neuron in NC (filled bar) and in ANCC (open bar). (D,E), examples of staining for synaptic puncta with antibodies against vGAT (orange) in NC (D) and ANCC (E). (F), Statistics for the number of vGATpuncta per neuron in NC (filled bar) and in ANCC (open bar). Asterisks indicate significant difference between respective groups, spacebar in pictures represents 100 μm.