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. 2015 Apr 9;6:135. doi: 10.3389/fgene.2015.00135

FIGURE 6.

FIGURE 6

Relative mtDNA content of different humanized yeast strains. The copy number of the mitochondria COX3 gene relative to that of the nuclear GAL4 gene by q-PCR was normalized to 1 for POLG(WT) haploid strain and POLG(WT)/POLG(WT) diploid strain as described previously (Qian et al., 2014).The genomic DNA was extracted from cells harvested at OD = 1.0. The data for S305R haploid and S305R/WT diploid cells were determined previously (Qian et al., 2014).