Table 1.

Effects of S305R and P1073L mutations on dATP incorporation.

	Kd,DNA nM	kpol s-1	Kd,app μM	kpol/Kd,app	μM-1s-1	kexos-1
WT∗	9.9 ± 2.1	30 ± 2	0.7 ± 0.14	43 ± 9	0.34 ± 0.04
S305R	100 ± 4	12.4 ± 0.75	1.2 ± 0.15	10 ± 1	0.11 ± 0.02
P1073L	10.2 ± 1.2	54.2 ± 11.8	1.5 ± 0.5	36.1 ± 7	0.28 ± 0.03

^*The kinetic parameters for WT enzyme were determined previously (Johnson et al., 2000; Batabyal et al., 2010).