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. 2015 Apr 9;6:276. doi: 10.3389/fmicb.2015.00276

FIGURE 2.

FIGURE 2

Adherence (A) and invasion (B) of wild-type S. Enteritidis and S. Enteritidis fimH::kan mutant strain to murine intestinal ICE-1 cells, in the absence (light gray bars) and presence of 0.2 M D-mannose (dark gray bars). Bacteria were grown statically at 37∘C in LB broth. For adhesion assay, bacteria (MOI 100:1), after five passages, were incubated for 1 h with cell monolayers growing in six-well plates. For invasion assay, cell monolayers growing in six-well plates were infected for 1 h with bacteria (MOI 10:1) after five passages, followed by an additional hour of incubation with culture media containing 50 μg/ml gentamicin. Data are means ± SD grown in triplicate assays, which were independently repeated at least three times.