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. 2015 Apr 9;6:276. doi: 10.3389/fmicb.2015.00276

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Copyright © 2015 Kuźmińska-Bajor Grzymajło Ugorski.

This is an open-access article distributed under the terms of the Creative Commons Attribution License(CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Secretion of IL-1b, IL-6, IL-10, and IL-12b by ICE-1 cells exposed to wild-type S. Enteritidis (light gray bars) and S. Enteritidis fimH::kan mutant strain (dark gray bars) at MOI 100:1. After 6 h incubation, the culture supernatants were collected and analyzed for the presence of interleukins by ELISA. Interleukin levels were normalized against unstimulated cells. Data represent the mean ± SD of four independent experiments, and were analyzed in each experiment to confirm accuracy and reproducibility of ELISA. Values marked with ^∗ differ significantly (P < 0.05).