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. Author manuscript; available in PMC: 2016 Mar 9.
Published in final edited form as: Dev Cell. 2015 Feb 19;32(5):574–588. doi: 10.1016/j.devcel.2015.01.014

Figure 3. SCML2 Regulates Differentiation of Spermatogenic Cells.

Figure 3

(A–C) Histological testicular sections stained with H&E. Polynucleated cells and elongated spermatids and are shown with arrows in (B) and (C), respectively. The scale bars represent 60 μm. Quantitative data of the percentage of tubules containing polynucleated cells are shown on the right in (B). Data are represented as mean ± SD (n = 5).

(D) DAPI staining of elongated spermatids in germ cell slides that maintain chromatin morphology. Because abnormal chromatin was consistently observed in the Scml2-KO elongated spermatids, the quantification was not included. The scale bars represent 10 μm.

(E) Histological section of epididymis stained with H&E. The scale bars represent 60 μm.

(F–K) Immunostaining of ZBTB16, STRA8, and SYCP3 in 8 weeks testes. The scale bars represent 40 μm. Quantitative data are shown in (G), (I), and (K). Data are represented as mean ± SD (n = 4).

(L and M) TUNEL staining using testicular sections. The scale bars represent 40 μm. Quantitative data are shown in (M). Data are represented as mean ± SD (n = 4).

(N) Immunostaining of cleaved caspase-3, γH2AX, and H1T in adult testes. Apoptotic cells are shown with arrows. The scale bars represent 40 μm. *p < 0.05, unpaired t test.