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. 2015 Apr 7;6:6498. doi: 10.1038/ncomms7498

Figure 3. Knockdown of FMO3 prevents the development of atherosclerosis in LIRKO mice.

Figure 3

At 4 to 6 weeks of age, male Flox and LIRKO mice were placed on an atherogenic Paigen diet, treated with control (Con) or FMO3 ASO for 16 weeks, and killed in the non-fasted state. Hepatic gene expression (a) was measured by real-time PCR. Hepatic protein levels (b,f) were measured by western blotting whole-cell lysates. (c,e) Plasma taken at the time of sacrifice was used to measure TMAO (c) or pooled (n=5–7 mice per group) and subjected to FPLC analysis (e). Total cholesterol (d) was measured after 12 weeks of ASO treatment and a 4-h fast. (g,h) Abdominal aortas were dissected and stained with Oil-Red-O (representative images shown in g). Lesion area was quantified and expressed as a percentage of the whole aorta (h). In the above in vivo experiments, data represent the mean and s.e.m.; n=5–13; *P<0.05 (Student's t-test) LIRKO mice treated with the control ASO versus Flox mice; #P<0.05 (Student's t-test) control ASO versus FMO3 ASO-treated LIRKO mice.