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. Author manuscript; available in PMC: 2015 Apr 9.
Published in final edited form as: J Cell Biochem. 2014 Mar;115(3):523–530. doi: 10.1002/jcb.24686

Figure 3. Low SLBP production rate is limited to early G1.

Figure 3

HeLa cells were synchronized by double thymidine block method and labeled with 35S-methionine for 15 minutes at G1 (2.5–3 hours after M/G1) or S phases after half hour incubation in methionine lacking media. A) Equal protein amounts of total lysates and B) immunoprecipitates with SLBP antibody or beads alone were run on SDS PAGE gel and detected with autoradiography. Cell cycle phases were confirmed by FACs analysis.