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. Author manuscript; available in PMC: 2015 Apr 9.
Published in final edited form as: J Cell Biochem. 2014 Mar;115(3):523–530. doi: 10.1002/jcb.24686

Figure 4. SLBP is degraded by proteasome in G1.

Figure 4

HeLa cells were synchronized by double thymidine block method and treated with A) Cycloheximide alone (100 μM), B) Cycloheximide and Mg132 C) Mg132 alone for 1 hour at indicated cell cycle phases. Western blot analysis is performed using anti-SLBP serum. The cross-reacting band recognized by anti-SLBP serum serves as loading control. Cell cycle phases were confirmed by FACs analysis.