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. Author manuscript; available in PMC: 2015 Apr 9.
Published in final edited form as: J Cell Biochem. 2014 Mar;115(3):523–530. doi: 10.1002/jcb.24686

Figure 5. S/G2 stable mutant SLBP is degraded by proteasome in G1.

Figure 5

HeLa cells stably expressing his-tagged mutant SLBP were synchronized by double thymidine method and treated with A) Cycloheximide alone B) Cycloheximide and Mg132 for 1 hour at indicated cell cycle phases. Cells were collected before and after treatment and western blot analysis is performed using anti- SLBP serum. Cell cycle phases were confirmed by FACs analysis.