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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Nutr Biochem. 2014 Dec 15;26(4):369–377. doi: 10.1016/j.jnutbio.2014.11.005

Figure 2. Clustering of lipid microdomains on murine B cells.

Figure 2

(A-D) Representative fluorescent images of lipid microdomains on purified, splenic B cells from SMAD3-/- mice fed CON, MO, EFO, or DFO diets. Cholera toxin subunit B conjugated to FITC was used to visualize GM1, an extensively used reporter of lipid rafts. Blinded scoring analysis of fluorescent lipid microdomains on cells as (E) clustered or (F) non-clustered. Data are represented as mean ± SEM, whereby 10 cells were scored per animal and n = 10-15 mice/group. Different letters denote statistically significant differences at the P < 0.05 level.