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Flow cytometry detection of Annexin V /PI-stained cells after treatment with different concentrations of 5-FU for 72 hours. The sum of events in the upper right quadrant (late apoptotic/necrotic cells) and in the low right quadrant (early apoptotic cells) divided by the total number of events acquired. Panels A to I represent the results induced by different doses of 5-FU from 0, 0.78, 1.56, 3.125, 6.25, 12.5, 25, 50 to100 μg/mL separately.
