Figure 3.

Isw2 and Ino80 function is independent of any single checkpoint factor downstream of RPA. (A) Schematic drawing of S-phase checkpoint pathway, which is divided into the DNA replication checkpoint (DRC) in pink, DNA damage checkpoint (DDC) in blue, and factors present in both pathways in yellow. (B) Bar graph showing the ratio of S-phase checkpoint activity in checkpoint mutants in the absence of ISW2 and NHP10 compared with the checkpoint mutant alone. The dashed red line indicates the expected S-phase checkpoint ratio if a given checkpoint protein is required for Isw2 and Ino80 function. (C) Rad53 ISA of mec1 sml1 and mec1 sml1 isw2 nhp10 cells released from G₁ into S phase containing 0.02% MMS. Time points in S phase are denoted in purple. (D) Corresponding FACS profiles of time points from the ISA, with profiles in purple denoting time points in S phase.