Figure 4.

Bulk levels of chromatin-bound checkpoint proteins are largely unchanged between WT and isw2 nhp10 cells. (A) FACS profiles of samples used for chromatin preparation in B during G₁ and S phase in 0.02% MMS. (B) Western blot of RPA and H2B levels from whole-cell extracts and fractionated chromatin of WT and isw2 nhp10 cells. Levels of RPA were normalized to H2B, and the quantitation is shown. (C) Chart graphing the SILAC mass spectrometry data of nuclear proteins from WT vs. isw2 nhp10 cells in middle S phase in 0.02% MMS. Total protein was normalized based on the total amount of histones.