Table 2. Reverse Genetic Approaches Available for Nasonia.
Approach | Works in Nasonia? | References |
---|---|---|
RNA interference | Yes (parental, embryonic, larval, adult) | Lynch and Desplan 2006; Abdel-Latief et al. 2008; Werren et al. 2009; Rosenberg et al. 2014 |
Morpholinos | Yes (embryonic) | Rosenberg et al. 2014 |
Germ-line transformation | Limited success | C. Desplan, personal communication |
CRISPR/Cas9 | Preliminary success | J. A. Lynch, personal observation |
TALENs | Yes | C. Desplan, personal communication |
Zinc finger nucleases | Not tested | n/a |