LR phenotypes of the male genitalia associated with mutations and overexpression of Myo31DF, Myo61F, and Myo95E. (A) Male genital plate of the wild-type (top left) and Myo31F homozygous mutant (top right) showing dextral (blue arrow) and sinistral (red arrow) rotations, respectively, viewed from the posterior during pupal stages. The spermiduct attached to the genital plate also showed dextral and sinistral looping around the hindgut in wild-type (bottom left) and Myo31F mutant homozygous flies (bottom right), respectively. A, anterior; D, dorsal; L, left; P, posterior; R, right; V, ventral. (B–E) Posterior views of the male genital plate of wild-type (B), Myo31DFK2 homozygote (C), Myo61F1 homozygote (D), and Myo31DFK2; Myo61F1 double-homozygote (E) flies. Dorsal is up. Insets at bottom right indicate the 360° dextral (blue), 180°–360° sinistral (orange), and 360° sinistral (red) rotations. White dotted lines indicate the midline of the genital plate. (F and G) Bar graphs showing the frequency (percentage) of dextral and sinistral rotation phenotypes observed in the male genital plate, color coded according to the categories shown at the bottom. Genotypes are indicated at left.