Table 1. Matrix examination of BiFC interactions among MSUD proteins.
SAD-1-YFPC | SAD-2-YFPC | YFPC-SAD-3 | DCL-1-YFPC | QIP-YFPC | YFPC-SMS-2 | |
---|---|---|---|---|---|---|
SAD-1-YFPN | ||||||
SAD-2-YFPN | +a | |||||
YFPN-SAD-3 | +b | +b | ||||
DCL-1-YFPN | + (A) | + (B) | − (C) | |||
QIP-YFPN | + (D) | + (E) | + (F) | − (G) | ||
YFPN-SMS-2 | + (H) | + (I) | + (J) | + (K) | +c |
A positive interaction between two proteins (i.e., one that leads to the reconstitution of the yellow fluorophore) is denoted by +. A negative result (−) may indicate that either the two proteins do not have intimate interaction or the BiFC assay is not sensitive enough to detect their weak/transient interaction. A–K in parentheses refer to Figure 1, A–K.